Peptide Chemistry
What is BPC-157? Sequence, formula and molecular weight
By the NexXGen Research Team · Updated November 14, 2026 · 4 min read
Key takeaways
- BPC-157 is a synthetic peptide made of 15 amino acids (a pentadecapeptide).
- In one-letter code its sequence is GEPPPGKPADDAGLV.
- Molecular formula C62H98N16O22, molecular weight about 1419.5 g/mol, CAS number 137525-51-0.
- A lab report gives purity by HPLC. Some also give the amount per vial or a mass for identity, so read what the report for your lot shows.
- For in-vitro, laboratory research use only.
BPC-157 is a synthetic pentadecapeptide: a single chain of 15 amino acids with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val. Its molecular formula is C62H98N16O22, its molecular weight is about 1419.5 g/mol, and its CAS number is 137525-51-0.
This guide gives laboratory staff the compound’s identifiers, the values to expect on an analytical report, and storage notes for the lyophilized powder. NexXGen Peptides lists it as lyophilized powder in two sizes: BPC-157 5 mg and BPC-157 10 mg.
What is the amino acid sequence of BPC-157?
The 15 residues run from glycine at the N-terminus to valine at the C-terminus. Four of them are proline, three in a row at positions 3 to 5. The chain has three acidic residues (one glutamic acid and two aspartic acids) and one basic residue (lysine), so it carries a net negative charge at neutral pH.
Two absences matter in the lab. There is no cysteine, so the peptide forms no disulfide bonds and stays a single linear chain. There is no tryptophan, tyrosine or phenylalanine either, which decides how it is detected on HPLC.
What are the molecular formula and molecular weight of BPC-157?
The formula counts 62 carbon, 98 hydrogen, 16 nitrogen and 22 oxygen atoms. The average molecular weight is about 1419.5 g/mol. Our product pages show 1419.54; small differences between sources come from rounding.
The monoisotopic mass is 1418.70 Da. A mass spectrum is checked against that figure, or against the average weight, depending on how the laboratory reports it.
Both figures describe the peptide alone. Synthetic peptides are often supplied as a salt, commonly acetate or trifluoroacetate, and the powder also holds some water. The mass of powder in a vial and the mass of peptide are therefore not the same number.
What should BPC-157 look like on an analytical report?
| Test | What it confirms | What to expect for this sequence |
|---|---|---|
| Mass spectrometry, when reported | Identity | A figure that fits the peptide, monoisotopic or average. Calculated electrospray ions: about m/z 1419.71 (one charge) and 710.36 (two charges) |
| HPLC with UV detection | Purity | One dominant peak, read at about 214 to 220 nm. With no aromatic residues, the peptide absorbs very little at 280 nm |
| Content, when reported | Amount of peptide in the vial | A figure in mg to compare with the size on the label |
| Net peptide content, when reported | Share of the powder that is peptide | A percentage below 100, because salt and water are part of the powder |
| Appearance | Physical form | Lyophilized powder, typically white to off-white |
The report for the lot in hand is the source of truth. Posted reports are on the Certificates of Analysis page.
How should lyophilized BPC-157 be stored?
Lyophilized peptide keeps best cold, dry and dark. Our product pages give -20°C for long-term storage, away from direct light and moisture.
Let a cold vial reach room temperature before you open it, so water from the air does not condense on the powder. Keep the vial sealed between openings.
What should a researcher check?
- Name and size. The compound and size on the report match the label.
- Lot number. The lot or batch on the vial is the one on the report.
- Purity. The report gives an HPLC purity figure for that lot.
- Mass, where the report gives one. It agrees with the values in the table above.
Research-use note
All NexXGen Peptides compounds are intended strictly for in-vitro research, laboratory and analytical purposes. They are not for human or animal use and must be handled only by qualified researchers aged 21 or older. Nothing in this article is medical advice.
Frequently asked questions
How many amino acids are in BPC-157?
Fifteen. A peptide of that length is called a pentadecapeptide.
What is the CAS number for BPC-157?
137525-51-0.
Is the molecular weight of BPC-157 the same as its monoisotopic mass?
No. The average molecular weight counts every isotope in its natural proportion. The monoisotopic mass counts only the most common isotope of each element, so it is slightly lower.
Why is BPC-157 read at 214 to 220 nm on HPLC?
Detection relies on the peptide bonds, which absorb in that range. The sequence has no aromatic residues to absorb at 280 nm.
Does BPC-157 contain disulfide bonds?
No. The sequence has no cysteine, so the peptide is one linear chain.